The alphamegalog of the cities of Florida lists the largest city in Florida for each letter of the alphabet. In this case, ‘largest’ is based on population within the city proper.

Alafaya
Brandon
Cape Coral
Davie
Egypt Lake Leto
Fort Lauderdale
Gainesville
Hialeah
Immokalee
Jacksonville
Kissimee
Lakeland
Miami
North Miami
Orlando
Port Saint Lucie
Quincy
Riverview
Saint Petersburg
Tampa
University
Valrico
West Palm Beach
(no X)
Yulee
Zephyrhills